Is there any way to get the single relais status by visual basic 6 ?

We assume that you would like to check the status of a relay?
This is not possible, as this is a ‘one-way’-card (output only).

So, it mean the board doesn’t provide any kind of feedback on triggering?

No, I repeat, this is a ‘one way’ output only board.
You must keep track of the status of the relays in your VB software.